Refine your search
Collections
Co-Authors
Journals
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Kumar, Satendar
- Characterization and Evaluation of Two Indigenous Bacillus thuringiensis Isolates against Helicoverpa armigera Hubner
Abstract Views :267 |
PDF Views:194
Authors
R. Rangeshwaran
1,
K. Veenakumari
1,
Pritam Karmakar
1,
K. Ashwitha
1,
G. Sivakumar
1,
Satendar Kumar
1
Affiliations
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 4 (2011), Pagination: 286-293Abstract
Two isolates of Bacillus thuringiensis isolated from dead lepidopteran larvae from a tea garden in Jorhat, Assam and one isolated from soil sample from Rajasthan, obtained in a nationwide screening program showed bipyramidal crystal morphology. These two isolates named as NBAII-BTAS and NBAII-BTG4 were characterized by their high level of toxicity against diamond back moth (Plutella xylostella). The PCR amplification of these two isolates revealed the expected size of the PCR product for cry1Aa, cry1Ab, cry1Ac, cry1E, cry1G, cry1I, and cry2 of 390 bps, 1111bps, 238 bps, 540 bps, 300 bps, 468 bps, and 1170 bps respectively. Purified cry proteins from each of these two cultures were subjected to SDS-PAGE analysis, where, two distinct bands of 130–140 Kda and 65 Kda corresponding to cry1 and cry2 proteins were observed. Toxicity studies was carried out using trypsin activated purified proteins against Helicoverpa armigera, where NBAII-BTG4 derived crystal proteins displayed more toxicity (0.93µg\ml) than NBAII-BTAS.Keywords
Cry Genes, PCR, Specific Cry Primers, Bipyramidal, Toxicity.References
- Aly, N. A. H. 2007. PCR detection of cry genes in local Bacillus thuringiensis Isolates, Australian Journal of Basic and Applied Sciences, 1: 461–466.
- Anitha, D., Senthil Kumar, N., Vijayan, D., Kunhikrishnan., Ajithkumar and Gurusubramanian, G. 2011. Characterization of Bacillus thuringiensis isolates and their differential toxicity against Helicoverpa armigera populations. Journal of Basic Microbiology, 51: 107–114.
- Avilla, C., Vargas-Osuna, E., González-Cabrera, J., Ferré, J. and González-Zamora, J. E. 2005. Toxicity of several ä-endotoxins of Bacillus thuringiensis against Helicoverpa armigera (Lepidoptera: Noctuidae) from Spain. Journal of Invertebrate Pathology, 90: 51–54.
- Avilla. C., Vargas-Osuna, E., González-Cabrera, J., Ferré, J. and González-Zamora, J. E. 2005. Toxicity of several endotoxins of Bacillus thuringiensis against Helicoverpa armigera (Lepidoptera: Noctuidae) from Spain. Journal of Invertebrate Pathology, 90: 51–54.
- Ben-Dov, E., Wang, Q., Zaritsky, A., Manasherob, R., Barak, Z., Schneider, B., Khamraev, A., Baizhanov, M., Glupov, V. and Margalith, Y. 1999. Multiplex PCR screening to detect cry 9 genes in Bacillus thuringiensis strain. Applied and Environmental Microbiology, 65: 3714–3716.
- Beron, C. M., Curatti, L. and Salerno, G. L. 2005. New strategy for identification of novel cry type genes from Bacillus thuringiensis strains. Applied and Environmental Microbiology, 71: 761–765.
- Beron, C. M. and Salerno, G. L. 2006. Characterization of Bacillus thuringiensis isolates from Argentina that are potentially useful in insect pest control. Biological Control, 51: 779–794.
- Bravo A., Sarabia, S., Lopez, L., Ontiveros, H., Abarca, C., Ortiz, A., Ortiz, M., Lina, L., Villalobos, F., Peña, G., Nuñez-Valdez, M. E., Soberón, M and Quintero, R. 1998. Characterization of cry genes in a mexican Bacillus thuringiensis strain collection. Applied and Environmental Microbiology, 64: 4965–4972.
- Carozzi, N. B., Kramer, V. C., Warren, G. W., Evola, S. and Koziel, M. G. 1991. Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Applied and Environmental Microbiology, 57: 3057–3061.
- Cerón, J. L., Covarrubias, R., Quintero, A., Ortiz, M., Ortiz, E. Aranda and Bravo, A. 1994. PCR analysis of the cry I insecticidal crystal family genes from Bacillus thuringiensis. Applied and Environmental Microbiology, 60: 353–356.
- Cerón, J. A., Ortiz, R., Quintero, L. G. and Bravo, A. 1995. Specific PCR primers directed to identify cryI and cry III genes within a Bacillus thuringiensis stray collection. Applied and Environment Microbiology, 61: 3826–3831.
- Chilcott, C. N. and Wigley, P. J. 1994. Isolation and toxicity of Bacillus thuringiensis from soil and insect habitats in New Zealand. Journal of Invertebrate Pathology, 61: 244–247.
- Gill, S. S., Cowles, E. A. and Pietrantinio, P. V. 1992. The mode of action of Bacillus thuringiensis endotoxins. Annual Review of Entomology, 37: 615–636.
- Höfte H. and Whiteley H. R., 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiological Reviews, 53: 242–255.
- Hofte, H., Van Rie, J., Jansens, S., Van Houtven, A., Vanderbruggen, H. and Vaeck, M. 1988. Monoclonal antibody analysis and insecticidal spectrum of three types of lepidopteran specific insecticidal crystal proteins of Bacillus thuringiensis. Applied and Environmental Microbiology, 54: 2010–2017.
- Konecka, E., Kaznowski, A., Zeimnicka, J., Zeminicki, K. and Paetz, H. 2007. Analysis of cry gene profiles in Bacillus thuringiensis strains isolated during epizootics in Cydia pomonella L., Current Microbiology, 55: 217–222.
- Kronstad, J. W. and Whiteley, H. R. 1986. Three classes of homologous Bacillus thuringiensis crystal protein genes. Gene, 43: 29–40.
- Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227: 680–685.
- Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. Journal of Biology and Chemistry, 193: 26.
- Mahadi, N. M., Hastowo, S., Lay, B. and Dean, D. H. 1998. Application of Multiplex P.C.R. for rapid determination of cryI gene profiles of new Bacillus thyringiensis isolates. Journal of Microbiology and Biotechnology, 8: 517–522.
- Martin, P. A. W., Travers, R. S. 1989. Worldwide abundance and distrbution of Bacillus thuringiensis isolates. Applied Environmental Microbiology, 55: 2437–2442.
- Morris, O. N., Converse, V., Kanagaratnam, P. and Cote, J. C. 1998. Isolation, characterization and culture of Bacillus thuringiensis from soil and dust from grain storage bins and their toxicity for Mamestra configurata (Lepidoptera: Noctuidae). Canadian Entomologist, 130: 515–537.
- Obeidat, M., Hassawi, D. and Ghabeish I. 2004. Characterization of Bacillus thuringiensis strains from Jordan and their toxicity to the Lepidoptera Ephestia kuehniella Zeller. African Journal of Biotechnology, 3: 622–626.
- Ohba, M. and Aizawa, K. 1986. Distribution of Bacillus thuringiensis in soils of Japan. Journal of Invertebrate Pathology, 47: 277–282.
- Porcar, M. and Jua.rez/Perez, V. 2003. PCR-based identification of Bacillus thuringiensis pesticidal crystal geners. FEMS Microbiology Reviews, 26: 419–432.
- Rosas-Garcia, N. M., Mireles-Martinez, M., Hernandez-Mendoza, J. L. and Ibarra, J. E. 2007. Screening of cry gene contents of Bacillus thuringiensis strains isolated from avocado orchards in Mexico, and their insecticidal activity towards Argyrotaenia sp. (Lepidoptera:Tortricidae) larvae. Journal of Applied Microbiology, 104: 224–230.
- Sambrook, J., Fritsch, E. F. and Mariatis, T. 1989. Molecular cloning: A Laboratory Manual, 2nd Ed., N. Y., Cold Spring Harbor Laboratory Press, 659p.
- Smith, R. A. and Couche, G. A. 1991. The phylloplane as a source of Bacillus thuringiensis. Applied and Environmental Microbiology, 57: 311–315.
- Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Fietelson, J., Ziegler, D. R. and Dean, D. H. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology and Molecular Biology Reviews. 62: 775–806.
- Shang Kuo, W., Lin, J. H., Tzeng, C. C., Kao, S. S. and Chak, K. F. 2000. Cloning of two new cry Genes from Bacillus thuringiensis subsp. wuhanensis strain. Current Microbiology, 40: 227–232.
- Thammasittirong, A. and Attathom, T. 2008. PCR-based method for the detection of cry genes inlocal isolates of Bacillus thuringiensis from Thailand. Journal Invertebrate Pathology, 98: 121–126.
- Travers, R. S., Martin, P. A. and Reichelderfer, C. F. 1987. Selective process for efficient isolation of soil Bacillus spp. Applied and Environmental Microbiology, 53: 1263- 1266
- Additives in Powder Based formulation for Enhanced Shelf Life of Pseudomonas fluorescens and Bacillus sp.
Abstract Views :289 |
PDF Views:148
Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN